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A Practical Guide to High Resolution Melt Analysis Genotyping
High resolution melt (HRM) analysis is a relatively new technique used in detecting small variations in DNA sequences between varying populations. Important applications of HRM include SNP analysis,...
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A Revolutionary Tool for 5 Minute Protein Visualization and Analysis
A short while ago, Bio-Rad Laboratories (who we all know as experts in electrophoresis and gel documentation) came out with a revolutionary new imaging system that allows for visualization of RNA, DNA...
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Working Range vs. Dynamic Range in Quantitative Immunoassays
For any quantitative immunoassay, the distinction between the dynamic range of an assay versus working range of an assay is an important consideration. Many believe the working range of an assay and...
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7 Great Resources for HRM Analysis
Bio-Rad Laboratories recently launched the Precision Melt Supermix, which is a high-perfomance supermix for both genotyping and epigenetic analyses. In honor of this launch, we invite you to review...
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Strategies for proteomics sample preparation
Two-dimensional (2-D) gel electrophoresis is a popular and proven separation technique for proteome analysis. The 2-D procedure is straightforward: Proteins are first separated according to their...
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Protein blotting guide for novice and advanced users
Protein blotting is a staple technique of most molecular biology and proteomics laboratories. In previous posts, we discussed topics such as semi-dry protein transfer and protein transfer methods, and...
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A primer on fluorescence detection
Yesterday we told you about how to get more data from your western blots by utilizing multiplex fluorescent detection. Today, we will provide you with a primer on fluorescent detection taken from the...
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Transfering high molecular weight proteins
We are all intimately familiar with protein blotting techniques which have been a cornerstone of the biochemicstry/biology lab for the past 30 years. As is well known, the efficiency of protein...
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A Sensitive Technique for Probing Small Differences in Copy Number Variation
Cytogenic studies over the past 50 years have hinted at the impact that copy number variations (CNVs) can have on phenotypic traits and disease susceptibility. Given the high incidence and clinical...
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Designing the Perfect Quantitative Western Blot
A methods article published yesterday provides a rigorous and concise workflow with specific instructions on how to produce and analyze quantitative data using western blot experiments. The paper,...
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